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Addgene

1154B_gFLE_pB_VTK_ActG
(Plasmid #187238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBVTK_ActG
  • Backbone manufacturer
    na
  • Total vector size (bp) 12559
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Femaleless
  • gRNA/shRNA sequence
    CGACGGCTCGTTCATCGCTG, ATCGAGCGCGTCGCCTGGTA
  • Species
    A. gambiae

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1154B_gFLE_pB_VTK_ActG was a gift from Omar Akbari (Addgene plasmid # 187238 ; http://n2t.net/addgene:187238 ; RRID:Addgene_187238)

  • For your References section:

    A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae. Smidler AL, Pai JJ, Apte RA, Sanchez C HM, Corder RM, Jeffrey Gutierrez E, Thakre N, Antoshechkin I, Marshall JM, Akbari OS. Sci Adv. 2023 Jul 7;9(27):eade8903. doi: 10.1126/sciadv.ade8903. Epub 2023 Jul 5. 10.1126/sciadv.ade8903 PubMed 37406109
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