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Addgene

pSBK.079
(Plasmid #187218)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187218 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3900
  • Total vector size (bp) 6072
  • Modifications to backbone
    swap Kan resistance marker for Amp resistance marker
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Retron Eco1 Reverse Transcriptase
  • Alt name
    Retron Ec86 RT
  • Species
    E. coli
  • Insert Size (bp)
    963
  • GenBank ID
    MFB61135.1
  • Promoter J23115

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CATACCGCGAAAGGTTTTGCG
  • 3′ sequencing primer TATGCGGCCGTGTACAATAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Cas1 + Cas2
  • Alt name
    Cas1+2
  • Species
    E. coli
  • Insert Size (bp)
    1204
  • Promoter T7/lac

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATCATCACCACAGCCAGGAT
  • 3′ sequencing primer ATGTGCTGGCGTTCAAATTTCGCAGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cas1 + Cas2 cloned from pCas1+2. pCas1+2 was a gift from Udi Qimron (Addgene plasmid # 72676 ; http://n2t.net/addgene:72676 ; RRID:Addgene_72676)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBK.079 was a gift from Seth Shipman (Addgene plasmid # 187218 ; http://n2t.net/addgene:187218 ; RRID:Addgene_187218)

  • For your References section:

    Recording gene expression order in DNA by CRISPR addition of retron barcodes. Bhattarai-Kline S, Lear SK, Fishman CB, Lopez SC, Lockshin ER, Schubert MG, Nivala J, Church GM, Shipman SL. Nature. 2022 Aug;608(7921):217-225. doi: 10.1038/s41586-022-04994-6. Epub 2022 Jul 27. 10.1038/s41586-022-04994-6 PubMed 35896746
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