pLV-DBNF-mCherry
(Plasmid #187195)

• Purpose: To express the mouse BDNF tagged with the FP mCherry
• Depositing Lab: Pantelis Tsoulfas
• Publication: Neurotrophin related plasmids (unpublished)

Backbone

• Vector backbone: pLenti-M2
• Backbone manufacturer: The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the
• Backbone size w/o insert (bp): 6767
• Total vector size (bp): 8249
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Low sodium LB
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BDNF
• Species: M. musculus (mouse)
• Insert Size (bp): 747
• Mutation: The codons have been optimized for better expression
• GenBank ID: NM_001048139
• Promoter: CMV
• Tag / Fusion Protein:
  • fluorescent protein mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI, XhoI (not destroyed)
• 3′ cloning site: NheI, XbaI, SalI, EcoRV (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry