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pLZRS-panTRK-CR1-EGFP
(Plasmid #187194)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLZRS
  • Total vector size (bp) 14774
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CR1 pan-TRK receptor
  • Alt name
    CR213
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3128
  • Promoter mo-MLV or MMLV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBMN 5'
  • 3′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Restriction digest confirmed the correct plasmid length (14,647 bp) with EBNA1 region present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZRS-panTRK-CR1-EGFP was a gift from Pantelis Tsoulfas (Addgene plasmid # 187194 ; http://n2t.net/addgene:187194 ; RRID:Addgene_187194)

  • For your References section:

    TrkA amino acids controlling specificity for nerve growth factor. O'Connell L, Hongo JA, Presta LG, Tsoulfas P. J Biol Chem. 2000 Mar 17;275(11):7870-7. doi: 10.1074/jbc.275.11.7870. 10.1074/jbc.275.11.7870 PubMed 10713102
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