pMSCV-FlagBcl10K31RK63R
(Plasmid #18719)

• Depositing Lab: Jon Ashwell
• Publication: Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8.

Backbone

• Vector backbone: pMSCVpuro
• Backbone manufacturer: BD Clontech
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B cell lymphoma 10
• Alt name: Bcl10
• Species: H. sapiens (human)
• Insert Size (bp): 750
• Mutation: Lysine 31 to arginine and Lysine 63 to arginine.
• GenBank ID: NM_003921
• Entrez Gene: BCL10 (a.k.a. CARMEN, CIPER, CLAP, IMD37, c-E10, mE10)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: pMSCV primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry