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Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H
(Plasmid #187183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHat2
  • Backbone size w/o insert (bp) 4373
  • Total vector size (bp) 5522
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ENSDARG00000024546
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1149
  • Mutation
    L32A, L33A
  • Entrez Gene
    pla2g4aa (a.k.a. PLA2G4A, cpla2, pla2g4)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H was a gift from Philipp Niethammer (Addgene plasmid # 187183 ; http://n2t.net/addgene:187183 ; RRID:Addgene_187183)

  • For your References section:

    A synergy between mechanosensitive calcium- and membrane-binding mediates tension-sensing by C2-like domains. Shen Z, Belcheva KT, Jelcic M, Hui KL, Katikaneni A, Niethammer P. Proc Natl Acad Sci U S A. 2022 Jan 4;119(1). pii: 2112390119. doi: 10.1073/pnas.2112390119. 10.1073/pnas.2112390119 PubMed 34969839
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