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Addgene

pCAG-H2B-mTurbo
(Plasmid #187178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187178 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    histone H2B fused with miniTurbo (BirA mutant)
  • Species
    Synthetic
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGCTTCTGGCGTGTGACC
  • 3′ sequencing primer AGCCACCACCTTCTGATAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-H2B-mTurbo was a gift from Isei Tanida (Addgene plasmid # 187178 ; http://n2t.net/addgene:187178 ; RRID:Addgene_187178)

  • For your References section:

    In-resin CLEM of Epon-embedded cells using proximity labeling. Sanada T, Yamaguchi J, Furuta Y, Kakuta S, Tanida I, Uchiyama Y. Sci Rep. 2022 Jul 1;12(1):11130. doi: 10.1038/s41598-022-15438-6. 10.1038/s41598-022-15438-6 PubMed 35778550
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