pCAG-mTurbo-G
(Plasmid
#187176)
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PurposeExpress miniTurbo under the control of CAG promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameminiTurbo (BirA mutant)
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SpeciesSynthetic
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Insert Size (bp)825
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GGCTTCTGGCGTGTGACC
- 3′ sequencing primer AGCCACCACCTTCTGATAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-mTurbo-G was a gift from Isei Tanida (Addgene plasmid # 187176 ; http://n2t.net/addgene:187176 ; RRID:Addgene_187176) -
For your References section:
In-resin CLEM of Epon-embedded cells using proximity labeling. Sanada T, Yamaguchi J, Furuta Y, Kakuta S, Tanida I, Uchiyama Y. Sci Rep. 2022 Jul 1;12(1):11130. doi: 10.1038/s41598-022-15438-6. 10.1038/s41598-022-15438-6 PubMed 35778550