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Addgene

FLAG-SENP8m
(Plasmid #18717)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18717 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PFLAG-CMV
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-SENP8m
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    640
  • Mutation
    C153A
  • GenBank ID
    AAH31411
  • Entrez Gene
    SENP8 (a.k.a. DEN1, NEDP1, PRSC2)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the FLAG tag does not appear to be in the correct reading frame for this gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-SENP8m was a gift from Edward Yeh (Addgene plasmid # 18717 ; http://n2t.net/addgene:18717 ; RRID:Addgene_18717)

  • For your References section:

    Neddylation of a breast cancer-associated protein recruits a class III histone deacetylase that represses NFkappaB-dependent transcription. Gao F, Cheng J, Shi T, Yeh ET. Nat Cell Biol. 2006 Oct . 8(10):1171-7. 10.1038/ncb1483 PubMed 16998474
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