pDEST_FLAG-HUWE1_C4341S
(Plasmid
#187156)
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Purposemammalian cell expression FLAG-HUWE C4341S
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHUWE1
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SpeciesH. sapiens (human)
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Insert Size (bp)13122
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MutationC4341S
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Entrez GeneHUWE1 (a.k.a. ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MRXST, MULE, URE-B1, UREB1)
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Tag
/ Fusion Protein
- 1xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST_FLAG-HUWE1_C4341S was a gift from Eric Fischer (Addgene plasmid # 187156 ; http://n2t.net/addgene:187156 ; RRID:Addgene_187156) -
For your References section:
Solenoid architecture of HUWE1 contributes to ligase activity and substrate recognition. Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer ES. Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26. 10.1016/j.molcel.2021.06.032 PubMed 34314700