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pDONR221-HUWE1_UIM-mut
(Plasmid #187147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HUWE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    13122
  • Mutation
    A1376G/S1382A
  • Entrez Gene
    HUWE1 (a.k.a. ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MRXST, MULE, URE-B1, UREB1)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-HUWE1_UIM-mut was a gift from Eric Fischer (Addgene plasmid # 187147 ; http://n2t.net/addgene:187147 ; RRID:Addgene_187147)

  • For your References section:

    Solenoid architecture of HUWE1 contributes to ligase activity and substrate recognition. Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer ES. Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26. 10.1016/j.molcel.2021.06.032 PubMed 34314700
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