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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3-RGS
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSENP3m
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SpeciesH. sapiens (human)
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Insert Size (bp)1770
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MutationC532A
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GenBank IDNC_000017 AAH80658
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Entrez GeneSENP3 (a.k.a. SMT3IP1, SSP3, Ulp1)
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Tag
/ Fusion Protein
- RGS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RGS-SENP3m was a gift from Edward Yeh (Addgene plasmid # 18714 ; http://n2t.net/addgene:18714 ; RRID:Addgene_18714) -
For your References section:
Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3. Gong L, Yeh ET. J Biol Chem. 2006 Jun 9. 281(23):15869-77. 10.1074/jbc.M511658200 PubMed 16608850
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/29/84/4d275024-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)