HA-Ubiquitin
(Plasmid #18712)

• Purpose: Mammalian expression of human ubiquitin with HA tag
• Depositing Lab: Edward Yeh
• Publication: Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-62.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ubiquitin
• Species: H. sapiens (human)
• Mutation: The HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence(full length UBB has three repeating ubiquitin coding sequences).
• GenBank ID: AB089617
• Entrez Gene: UBB (a.k.a. HEL-S-50)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 165 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid grows slowly. We encourage requesting scientists to grow for a longer period of time than normal. Please note that this plasmid runs as a dimer (>10kb) or multimer.