pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP
(Plasmid
#187113)
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PurposeProtein Expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6P-1
- Backbone size w/o insert (bp) 5701
- Total vector size (bp) 6044
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameENSG00000101199
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SpeciesH. sapiens (human)
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Entrez GeneARFGAP1 (a.k.a. ARF1GAP, HRIHFB2281)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer 5GEX
- 3′ sequencing primer 3GEX (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP was a gift from Philipp Niethammer (Addgene plasmid # 187113 ; http://n2t.net/addgene:187113 ; RRID:Addgene_187113) -
For your References section:
A synergy between mechanosensitive calcium- and membrane-binding mediates tension-sensing by C2-like domains. Shen Z, Belcheva KT, Jelcic M, Hui KL, Katikaneni A, Niethammer P. Proc Natl Acad Sci U S A. 2022 Jan 4;119(1). pii: 2112390119. doi: 10.1073/pnas.2112390119. 10.1073/pnas.2112390119 PubMed 34969839
Map uploaded by the depositor.
