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Addgene

pET51b_SNAP-ecDHFR
(Plasmid #187108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET51b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SNAP-ecDHFR
  • Species
    Synthetic; DHFR from E. coli
  • Promoter T7
  • Tags / Fusion Proteins
    • Strep (N terminal on insert)
    • Hisx10 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET51b_SNAP-ecDHFR was a gift from Kai Johnsson (Addgene plasmid # 187108 ; http://n2t.net/addgene:187108 ; RRID:Addgene_187108)

  • For your References section:

    Environmentally Sensitive Color-Shifting Fluorophores for Bioimaging. Wang L, Hiblot J, Popp C, Xue L, Johnsson K. Angew Chem Int Ed Engl. 2020 Dec 1;59(49):21880-21884. doi: 10.1002/anie.202008357. Epub 2020 Sep 28. 10.1002/anie.202008357 PubMed 32762146
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