pAAV-EF1a-DIO-EGFP-WPRE
(Plasmid
#187103)
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PurposeDouble floxed EGFP under the control of EF1a promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
- Promoter EF1a
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer EF1a
- 3′ sequencing primer WPRE (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-DIO-EGFP-WPRE was a gift from Yongling Zhu (Addgene plasmid # 187103 ; http://n2t.net/addgene:187103 ; RRID:Addgene_187103) -
For your References section:
Intersectional mapping of multi-transmitter neurons and other cell types in the brain. Xu J, Jo A, DeVries RP, Deniz S, Cherian S, Sunmola I, Song X, Marshall JJ, Gruner KA, Daigle TL, Contractor A, Lerner TN, Zeng H, Zhu Y. Cell Rep. 2022 Jul 5;40(1):111036. doi: 10.1016/j.celrep.2022.111036. 10.1016/j.celrep.2022.111036 PubMed 35793636
