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pCMV/myc/Nuc-mutant-hogg1
(Plasmid #18710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMCV/myc/nuc
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8-oxoguanine DNA glycosylase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1035
  • Mutation
    Amino Acid 229 Arginine changed to Glutamine
  • GenBank ID
    NM_002542
  • Entrez Gene
    OGG1 (a.k.a. HMMH, HOGG1, MUTM, OGH1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer cgcaaatgggcggtag
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV/myc/Nuc-mutant-hogg1 was a gift from David Sidransky (Addgene plasmid # 18710 ; http://n2t.net/addgene:18710 ; RRID:Addgene_18710)

  • For your References section:

    Targeting of mutant hogg1 in mammalian mitochondria and nucleus: effect on cellular survival upon oxidative stress. Chatterjee A, Mambo E, Zhang Y, Deweese T, Sidransky D. BMC Cancer. 2006 . 6():235. 10.1186/1471-2407-6-235 PubMed 17018150
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