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pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR
(Plasmid #187095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 5379
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pre-SPHERE-sfmTurquoise2-iGluSnFR
  • Species
    Synthetic
  • Insert Size (bp)
    2046
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry-P2A (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Loren Looger, Robert Campbell and Bong-Kiun Kaang, Addgene #106202, #107337, #107335, #111579

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR was a gift from Kotaro Oka (Addgene plasmid # 187095 ; http://n2t.net/addgene:187095 ; RRID:Addgene_187095)

  • For your References section:

    Genetically encoded sensors for analysing neurotransmission among synaptically-connected neurons. Shindo Y, Ashida K, Masamoto K, Takuwa H, Takahashi M, Higuchi M, Ide R, Hotta K, Oka K. bioRxiv 2022 10.1101/2022.04.03.486903
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