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Addgene

pBAD33(A-H*HA-N)
(Plasmid #187086)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187086 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nuoA-N
  • Species
    E. coli
  • Tag / Fusion Protein
    • HA tags nuoN, nuoH (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD33(A-H*HA-N) was a gift from Steven Vik (Addgene plasmid # 187086 ; http://n2t.net/addgene:187086 ; RRID:Addgene_187086)

  • For your References section:

    Analysis of the assembly pathway for membrane subunits of Complex I reveals that subunit L (ND5) can assemble last in E. coli. Zhang F, Vik SB. BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2021.100027. Epub 2021 Oct 17. 10.1016/j.bbadva.2021.100027 PubMed 35814529
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