pFV2-HA
(Plasmid #187082)

• Purpose: Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)
• Depositing Lab: Steven Vik
• Publication: Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1129-34. doi: 10.1016/j.bbabio.2009.03.022. Epub 2009 Apr 9.

Backbone

• Vector backbone: unknown
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: atpBEFHAGDC
• Species: E. coli
• Mutation: All Cys codons changed to Ala.
• Tags / Fusion Proteins:
  • 6-His beta subunit (atpD) (N terminal on insert)
  • HA tag subunit a (atpB) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a N419K mutation in ATPase alpha and a D6E mutation in ATPase gamma. These mutation are not known to affect plasmid function.