Human Metabolic Gene CRISPRa sgRNA Library
(Pooled Library #187080)

• Purpose:

  This lentiviral CRISPRa library targets 2,989 human metabolic genes for activation of gene expression.

• Vector Backbone:

  lentiGuide-Puro (Plasmid #52963) - does not express Cas9

• Depositing Labs: Kivanc Birsoy
• Publication: Unlu et al. Mol Cell. 2022 Aug 4;82(15):2832-2843.e7. doi: 10.1016/j.molcel.2022.05.024.

Library Details

• Species: Human
• Genes targeted: 2,989 metabolic genes
• gRNAs: 32,460
• Controls: 49 non-targeting sgRNAs
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: 20 µL
• Concentration: 25 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (DOCX, 16 KB)
  • Primers and PCR Protocol (DOCX, 16 KB)
• Depositor Data:
  • List of gRNA sequences (XLSX, 1.1 MB)
  • List of NGS Read Counts (TXT, 652 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the publication (Unlu et al. 2022) for more detailed information. The preprint is also available on bioRxiv .