pEZYpci
(Plasmid #18707)

• Purpose: (Empty Backbone) pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia
• Depositing Lab: Yu-Zhu Zhang
• Publication: Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Backbone

• Vector backbone: pPIC3.5K
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 11282
• Modifications to backbone: Gateway cassette was inserted into original vector to create the pEZYpci Gateway Destination vector
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DB3.1
• Growth instructions: E.coli strain DB3.1 @ 30C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pPIC3.5K Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction for integration of gene of interest into Pichia.