pEZYshuttle
(Plasmid #18703)

• Purpose: (Empty Backbone) pShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system
• Depositing Lab: Yu-Zhu Zhang
• Publication: Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Backbone

• Vector backbone: pShuttle-CMV
• Backbone manufacturer: Bert Vogelstein (Addgene plasmid# 16403)
• Backbone size (bp): 9772
• Modifications to backbone: Gateway cassette was inserted into original vector to create the pEZYshuttle Gateway Destination vector
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DB3.1
• Growth instructions: E.coli strain DB3.1, at 30C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pShuttle-CMV Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a shuttle vector for the AdEasy adenoviral vector system.