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pcDNA3.1-IDO1-p2a-eGFP
(Plasmid #187026)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187026 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)-P2A-eGFP-C
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Indoleamine 2,3-dioxygenase 1
  • Alt name
    IDO1
  • Alt name
    Indoleamine-pyrrole 2,3-dioxygenase
  • Alt name
    INDO
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_002164.5
  • Entrez Gene
    IDO1 (a.k.a. IDO, IDO-1, INDO)
  • Promoter CMV
  • Tag / Fusion Protein
    • P2A-GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic gene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-IDO1-p2a-eGFP was a gift from Geert van den Bogaart (Addgene plasmid # 187026 ; http://n2t.net/addgene:187026 ; RRID:Addgene_187026)

  • For your References section:

    Mitochondrial interaction of fibrosis-protective 5-methoxy tryptophan enhances collagen uptake by macrophages. Maassen S, Warner H, Ioannidis M, Jansma J, Markus H, El Aidy S, Chiara MD, Chiara JL, Maierhofer L, Weavers H, van den Bogaart G. Free Radic Biol Med. 2022 Aug 1;188:287-297. doi: 10.1016/j.freeradbiomed.2022.06.235. Epub 2022 Jun 23. 10.1016/j.freeradbiomed.2022.06.235 PubMed 35753585
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