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Addgene

EGFP-Tau(T217A)
(Plasmid #187025)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187025 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 7425
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAPT
  • Alt name
    Tau
  • Alt name
    This T217A tau construct contains human four-repeat tau and two inserts in N-terminal sequences with point mutation at T217A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2055
  • Mutation
    Changed Threonine 217 to Alanine
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.04.18.488657 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-Tau(T217A) was a gift from George Bloom (Addgene plasmid # 187025 ; http://n2t.net/addgene:187025 ; RRID:Addgene_187025)
  • For your References section:

    Localization, induction, and cellular effects of tau phosphorylated at threonine 217. Rajbanshi B, Guruacharya A, Mandell JW, Bloom GS. Alzheimers Dement. 2023 Jan 12. doi: 10.1002/alz.12892. 10.1002/alz.12892 PubMed 36633254