EGFP-Tau(T217A)
(Plasmid #187025)

• Purpose: Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter
• Depositing Lab: George Bloom
• Publication: Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.1002/alz.12892.

Backbone

• Vector backbone: pRK5
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 7425
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAPT
• Alt name: Tau
• Alt name: This T217A tau construct contains human four-repeat tau and two inserts in N-terminal sequences with point mutation at T217A
• Species: H. sapiens (human)
• Insert Size (bp): 2055
• Mutation: Changed Threonine 217 to Alanine
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pRK5-EGFP-Tau (T217A).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.04.18.488657 for bioRxiv preprint.