Skip to main content
Addgene

GFP-KASH2
(Plasmid #187017)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187017 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPB
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KASH2
  • Species
    M. musculus (mouse)
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Inducible construct via doxycycline addition

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-KASH2 was a gift from Jan Lammerding (Addgene plasmid # 187017 ; http://n2t.net/addgene:187017 ; RRID:Addgene_187017)

  • For your References section:

    Mutant lamins cause nuclear envelope rupture and DNA damage in skeletal muscle cells. Earle AJ, Kirby TJ, Fedorchak GR, Isermann P, Patel J, Iruvanti S, Moore SA, Bonne G, Wallrath LL, Lammerding J. Nat Mater. 2019 Dec 16. pii: 10.1038/s41563-019-0563-5. doi: 10.1038/s41563-019-0563-5. 10.1038/s41563-019-0563-5 PubMed 31844279
Related items:
Commonly requested with: