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Addgene

pNL-CEF-IgG1-IRES-GFP
(Plasmid #187008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187008 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNL-CEF
  • Backbone size w/o insert (bp) 11238
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2385

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNL-CEF-IgG1-IRES-GFP was a gift from Hermann Eibel (Addgene plasmid # 187008 ; http://n2t.net/addgene:187008 ; RRID:Addgene_187008)

  • For your References section:

    BAFFR activates PI3K/AKT signaling in human naive but not in switched memory B cells through direct interactions with B cell antigen receptors. Sevdali E, Block V, Lataretu M, Li H, Smulski CR, Briem JS, Heitz Y, Fischer B, Ramirez NJ, Grimbacher B, Jack HM, Voll RE, Holzer M, Schneider P, Eibel H. Cell Rep. 2022 Jun 28;39(13):111019. doi: 10.1016/j.celrep.2022.111019. 10.1016/j.celrep.2022.111019 PubMed 35767961
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