Phat2-dr-cPla2-C2-EGFP-6H
(Plasmid
#187001)
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PurposeProtein Expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHat2
- Backbone size w/o insert (bp) 4373
- Total vector size (bp) 5522
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameENSDARG00000024546
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1149
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Entrez Genepla2g4aa (a.k.a. PLA2G4A, cpla2, pla2g4)
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Phat2-dr-cPla2-C2-EGFP-6H was a gift from Philipp Niethammer (Addgene plasmid # 187001 ; http://n2t.net/addgene:187001 ; RRID:Addgene_187001) -
For your References section:
A synergy between mechanosensitive calcium- and membrane-binding mediates tension-sensing by C2-like domains. Shen Z, Belcheva KT, Jelcic M, Hui KL, Katikaneni A, Niethammer P. Proc Natl Acad Sci U S A. 2022 Jan 4;119(1). pii: 2112390119. doi: 10.1073/pnas.2112390119. 10.1073/pnas.2112390119 PubMed 34969839
Map uploaded by the depositor.
