pEZYflag
(Plasmid #18700)

• Purpose: (Empty Backbone) pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells
• Depositing Lab: Yu-Zhu Zhang
• Publication: Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Backbone

• Vector backbone: pFlag-CMV-2
• Backbone manufacturer: Sigma
• Backbone size (bp): 6932
• Modifications to backbone: Gateway cassette was inserted into original vector to create the pEZYflag Gateway Destination vector
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DB3.1
• Growth instructions: E.coli strain DB3.1 @ 30C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag.