BA14
(Bacterial strain
#186997)
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PurposeDeletion of the nuo operon
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 186997 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)BA14
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Growth instructionsThis strain grows best at 37˚C on LB. It grows very poorly in M63 + 0.2% acetate.
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebglR, thi-1, rel-1 HfrPO1, ∆nuoA-N
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SpeciesE. coli
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Parent strain is 1100, from Humbert et al. 1983 J Bacteriol. doi.org/10.1128/jb.153.1.416-422.1983
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BA14 was a gift from Steven Vik (Addgene plasmid # 186997) -
For your References section:
Construction of a deletion strain and expression vector for the Escherichia coli NADH:ubiquinone oxidoreductase (Complex I). Amarneh B, De Leon-Rangel J, Vik SB. Biochim Biophys Acta. 2006 Dec;1757(12):1557-60. doi: 10.1016/j.bbabio.2006.08.003. Epub 2006 Aug 10. 10.1016/j.bbabio.2006.08.003 PubMed 16979134