pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide
(Plasmid
#186993)
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PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecas7-11-s1006-gggs-d122, non-targeting crRNA guide
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gRNA/shRNA sequencecrRNA: gTTGatgtcacggaacGGTAATGCCTGGCTTGTCGACGCATAGTCTGttttttt
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SpeciesD. ishitomonii
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Mutations1006-gggs-d1221 deletion
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 186993 ; http://n2t.net/addgene:186993 ; RRID:Addgene_186993) -
For your References section:
Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex. Kato K, Zhou W, Okazaki S, Isayama Y, Nishizawa T, Gootenberg JS, Abudayyeh OO, Nishimasu H. Cell. 2022 May 25. pii: S0092-8674(22)00581-5. doi: 10.1016/j.cell.2022.05.003. 10.1016/j.cell.2022.05.003 PubMed 35643083