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pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc
(Plasmid #186987)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186987 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cas7-11-s1006-gggs-d122, Gluc crRNA guide
  • gRNA/shRNA sequence
    crRNA: gTTGatgtcacggaacTGCAGCCAGCTTTCCGGGCATTGGCTTCCATttttttt
  • Species
    D. ishitomonii
  • Mutation
    s1006-gggs-d1221 deletion

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains an 18bp deletion in the backbone immediately downstream of the crRNA sequence compared to the depositor's provided sequence. This is not known to affect plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 186987 ; http://n2t.net/addgene:186987 ; RRID:Addgene_186987)

  • For your References section:

    Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex. Kato K, Zhou W, Okazaki S, Isayama Y, Nishizawa T, Gootenberg JS, Abudayyeh OO, Nishimasu H. Cell. 2022 May 25. pii: S0092-8674(22)00581-5. doi: 10.1016/j.cell.2022.05.003. 10.1016/j.cell.2022.05.003 PubMed 35643083
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