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Addgene

MSCV-TIMP1
(Plasmid #186979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Vector type
    Retroviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tissue inhibitor of metalloproteinase 1
  • Alt name
    TIMP1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    643
  • GenBank ID
    NM_001044384.1
  • Entrez Gene
    Timp1 (a.k.a. Clgi, EPA, TIMP-1, TPA-S1, Timp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer ccgCTCGAGcagagcagataccatgatg
  • 3′ sequencing primer ccgGAATTCgaaggcttcaggtcatcg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-TIMP1 was a gift from Yong-Rui Zou (Addgene plasmid # 186979 ; http://n2t.net/addgene:186979 ; RRID:Addgene_186979)

  • For your References section:

    Host factor TIMP1 sustains long-lasting myeloid-biased hematopoiesis after severe infection. Song T, Yao Y, Papoin J, Sherry B, Diamond B, Gu H, Blanc L, Zou YR. J Exp Med. 2023 Dec 4;220(12):e20230018. doi: 10.1084/jem.20230018. Epub 2023 Oct 18. 10.1084/jem.20230018 PubMed 37851372
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