pLenti_CMV_pHoran4
(Plasmid
#186975)
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PurposeExpresses fluorescent pH sensor in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFCMV
- Total vector size (bp) 8451
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepHoran4
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Insert Size (bp)747
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gtgagcaagggcgaggag (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.02.07.527404v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_CMV_pHoran4 was a gift from Adam Cohen (Addgene plasmid # 186975 ; http://n2t.net/addgene:186975 ; RRID:Addgene_186975) -
For your References section:
Diminishing neuronal acidification by channelrhodopsins with low proton conduction. Hayward RF, Brooks FP 3rd, Yang S, Gao S, Cohen AE. eLife. 2023 Oct 6;12:RP86833. doi: 10.7554/eLife.86833. 10.7554/eLife.86833 PubMed 37801078
