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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemonomeric EGFP
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Alt namemEGFP
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Insert Size (bp)700
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MutationA206K mutation
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEGFP was a gift from Karel Svoboda (Addgene plasmid # 18696 ; http://n2t.net/addgene:18696 ; RRID:Addgene_18696) -
For your References section:
The spread of Ras activity triggered by activation of a single dendritic spine. Harvey CD, Yasuda R, Zhong H, Svoboda K. Science. 2008 Jul 4. 321(5885):136-40. 10.1126/science.1159675 PubMed 18556515
Map uploaded by the depositor.
