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Addgene

pJW2341
(Plasmid #186950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone size w/o insert (bp) 2360
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
    1269

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW2341 was a gift from Jordan Ward (Addgene plasmid # 186950 ; http://n2t.net/addgene:186950 ; RRID:Addgene_186950)

  • For your References section:

    The mIAA7 degron improves auxin-mediated degradation in C. elegans. Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, Boxem M. G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 10.1093/g3journal/jkac222. 10.1093/g3journal/jkac222 PubMed 36029236
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