MARCKS(1-40)-paGFP
(Plasmid #18695)

• Depositing Lab: Karel Svoboda
• Publication: Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MARCKS(1-40)
• Species: H. sapiens (human)
• Insert Size (bp): 120
• Mutation: A206K mutation in paGFP
• GenBank ID: DQ341274
• Entrez Gene: MARCKS (a.k.a. 80K-L, MACS, PKCSL, PRKCSL)
• Tag / Fusion Protein:
  • photoactivatable GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: J. Lippincott-Schwartz (paGFP)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry