paGFP-HRas G12V
(Plasmid #18694)

• Depositing Lab: Karel Svoboda
• Publication: Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H-Ras G12V
• Alt name: HRas G12V
• Alt name: HRas
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Mutation: A206K mutation in paGFP G12V mutation in HRas
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Tag / Fusion Protein:
  • photoactivatable GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Matsuda (H-Ras) J. Lippincott-Schwartz (paGFP)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry