pBlueScript II SK-SNAP donor for H3f3b
(Plasmid
#186933)
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PurposeGenomic targeting of SNAP tag at H3f3b C-terminal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript II SK
- Backbone size w/o insert (bp) 2958
- Total vector size (bp) 5226
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNAP donor for H3f3b
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SpeciesM. musculus (mouse)
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Insert Size (bp)2268
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Entrez GeneH3f3b (a.k.a. 9430068D06Rik, H3-3a, H3-3b, H3.3B)
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Tag
/ Fusion Protein
- SNAP tag
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer KS primer: CGAGGTCGACGGTATCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBlueScript II SK-SNAP donor for H3f3b was a gift from Zhiguo Zhang (Addgene plasmid # 186933 ; http://n2t.net/addgene:186933 ; RRID:Addgene_186933) -
For your References section:
Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions. Xu X, Duan S, Hua X, Li Z, He R, Zhaang Z. Nat Commun. 2022 May 6;13(1):2514. doi: 10.1038/s41467-022-30298-4. 10.1038/s41467-022-30298-4 PubMed 35523900