pBlueScript II SK-SNAP donor for Hist1h3g
(Plasmid
#186932)
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PurposeGenomic targeting of SNAP tag at Hist1h3g C-terminal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript II SK
- Backbone size w/o insert (bp) 2958
- Total vector size (bp) 5175
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNAP donor for Hist1h3g
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SpeciesM. musculus (mouse)
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Insert Size (bp)2217
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Entrez GeneH3c8 (a.k.a. H3.1-221, H3c1, H3c10, H3c11, Hist1h3g, M32460)
- Promoter T7
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Tag
/ Fusion Protein
- SNAP
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer KS primer: CGAGGTCGACGGTATCG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBlueScript II SK-SNAP donor for Hist1h3g was a gift from Zhiguo Zhang (Addgene plasmid # 186932 ; http://n2t.net/addgene:186932 ; RRID:Addgene_186932) -
For your References section:
Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions. Xu X, Duan S, Hua X, Li Z, He R, Zhaang Z. Nat Commun. 2022 May 6;13(1):2514. doi: 10.1038/s41467-022-30298-4. 10.1038/s41467-022-30298-4 PubMed 35523900