InterCatch
(Plasmid
#186906)
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PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance gene
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSBbi-GP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTfR-SpyCatcher003-sfCherry1-10
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
InterCatch was a gift from Taekjip Ha & Allen Liu (Addgene plasmid # 186906 ; http://n2t.net/addgene:186906 ; RRID:Addgene_186906) -
For your References section:
Engineering Functional Membrane-Membrane Interfaces by InterSpy. Moghimianavval H, Patel C, Mohapatra S, Hwang SW, Kayikcioglu T, Bashirzadeh Y, Liu AP, Ha T. Small. 2022 May 26:e2202104. doi: 10.1002/smll.202202104. 10.1002/smll.202202104 PubMed 35618485