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Addgene

sCatch-GFP-6xHIS
(Plasmid #186904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP-SpyCatcher003-sfCherry1-10-6xHIS
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • sfGFP (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sCatch-GFP-6xHIS was a gift from Taekjip Ha & Allen Liu (Addgene plasmid # 186904 ; http://n2t.net/addgene:186904 ; RRID:Addgene_186904)

  • For your References section:

    Engineering Functional Membrane-Membrane Interfaces by InterSpy. Moghimianavval H, Patel C, Mohapatra S, Hwang SW, Kayikcioglu T, Bashirzadeh Y, Liu AP, Ha T. Small. 2022 May 26:e2202104. doi: 10.1002/smll.202202104. 10.1002/smll.202202104 PubMed 35618485
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