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Addgene

Cdk6-DN-HA
(Plasmid #1869)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-neo-Bam
  • Backbone size w/o insert (bp) 6550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdk6
  • Alt name
    cyclin-dependent kinase 6
  • Alt name
    PLSTIRE
  • Species
    H. sapiens (human)
  • Mutation
    Dominant negative, D to N in conserved KLAD*FGLAR stretch; also D224Y
  • Entrez Gene
    CDK6 (a.k.a. MCPH12, PLSTIRE)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See scanned map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cdk6-DN-HA was a gift from Sander van den Heuvel (Addgene plasmid # 1869 ; http://n2t.net/addgene:1869 ; RRID:Addgene_1869)

  • For your References section:

    Distinct roles for cyclin-dependent kinases in cell cycle control. van den Heuvel S, Harlow E. Science 1993 Dec 24;262(5142):2050-4. 10.1126/science.8266103 PubMed 8266103
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