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pRRL-mouse cGAS #2-gRNA-Cas9-Puro
(Plasmid #186891)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186891 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL-Cas9-Puro
  • Backbone size w/o insert (bp) 11892
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    cGAS gRNA#2
  • gRNA/shRNA sequence
    GGCAGCCCAGAGCGCCGCGA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    20
  • Entrez Gene
    Cgas (a.k.a. E330016A19Rik, Mb21d1)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AACGGATCTCGACGGTATCGG
  • 3′ sequencing primer AACTTGCTATTTCTAGCTCTAAAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRL-mouse cGAS #2-gRNA-Cas9-Puro was a gift from Jonathan Kagan (Addgene plasmid # 186891 ; http://n2t.net/addgene:186891 ; RRID:Addgene_186891)

  • For your References section:

    Species-specific self-DNA detection mechanisms by mammalian cyclic GMP-AMP synthases. Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande AA, Hancks DC, Kranzusch PJ, Kagan JC. Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20. 10.1126/sciimmunol.abp9765 PubMed 36662885
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