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pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM
(Plasmid #186889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV GFP Puro
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman (Addgene plasmid # 17448)
  • Backbone size w/o insert (bp) 7867
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Orangutan cGAS truncation mutant (158-521 a.a.) with N-terminal FLAG tag and C-terminal MAVS transmembrane domain fusion
  • Alt name
    cGAS
  • Alt name
    cGASdeltaN
  • Species
    Synthetic; Pongo abelii
  • Insert Size (bp)
    1236
  • Mutation
    N-terminal truncation, MAVS transmembrane domain fusion
  • Entrez Gene
    CGAS (a.k.a. CR201_G0047729, MB21D1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains Kozak sequence (gccacc) before start codon. OMM: Outer Mitochondrial Membrane. Please visit https://www.biorxiv.org/content/10.1101/2022.03.09.483681v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM was a gift from Jonathan Kagan (Addgene plasmid # 186889 ; http://n2t.net/addgene:186889 ; RRID:Addgene_186889)

  • For your References section:

    Species-specific self-DNA detection mechanisms by mammalian cyclic GMP-AMP synthases. Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande AA, Hancks DC, Kranzusch PJ, Kagan JC. Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20. 10.1126/sciimmunol.abp9765 PubMed 36662885
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