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Addgene

pCAGGS-FLAG-BMAL1-S513A
(Plasmid #186831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Total vector size (bp) 6701
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bmal1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1878
  • Mutation
    changed Serine 513 to Alanine
  • GenBank ID
    NM_007489.4
  • Entrez Gene
    Bmal1 (a.k.a. Arnt3, Arntl, BMAL1b, MOP3, bHLHe5, bmal1b')
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-FLAG-BMAL1-S513A was a gift from Hung-Chun Chang (Addgene plasmid # 186831 ; http://n2t.net/addgene:186831 ; RRID:Addgene_186831)

  • For your References section:

    ISX-9 potentiates CaMKIIdelta-mediated BMAL1 activation to enhance circadian amplitude. Li H, Ou J, Li Y, Xu N, Li Q, Wu P, Peng C, Tang YC, Chang HC. Commun Biol. 2022 Jul 28;5(1):750. doi: 10.1038/s42003-022-03725-x. 10.1038/s42003-022-03725-x PubMed 35902736
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