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Addgene

pGL3-Camk2b
(Plasmid #186819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5892
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Camk2b promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1089
  • Entrez Gene
    Camk2b (a.k.a. CaMKII)
  • Promoter Camk2b promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CTTACGCGTATCCAGAAGATCGCCGAACAG
  • 3′ sequencing primer GCGAGATCTGCTCGCTCTGTCCCC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Camk2b was a gift from Hung-Chun Chang (Addgene plasmid # 186819 ; http://n2t.net/addgene:186819 ; RRID:Addgene_186819)

  • For your References section:

    ISX-9 potentiates CaMKIIdelta-mediated BMAL1 activation to enhance circadian amplitude. Li H, Ou J, Li Y, Xu N, Li Q, Wu P, Peng C, Tang YC, Chang HC. Commun Biol. 2022 Jul 28;5(1):750. doi: 10.1038/s42003-022-03725-x. 10.1038/s42003-022-03725-x PubMed 35902736
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