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pClgn-Tesmin-BioID2-3xFLAG
(Plasmid #186816)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186816 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-Tesmin-BioID2-3xFLAG
  • Backbone manufacturer
    Masahito Ikawa
  • Backbone size w/o insert (bp) 7362
  • Total vector size (bp) 5981
  • Modifications to backbone
    CAG promoter was replaced with mouse Clgn promoter
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tesmin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    323
  • Entrez Gene
    Tesmin (a.k.a. Mtl5)
  • Promoter mouse Clgn promoter
  • Tag / Fusion Protein
    • BioID2-3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer caggaaacagctatgac
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClgn-Tesmin-BioID2-3xFLAG was a gift from Masahito Ikawa (Addgene plasmid # 186816 ; http://n2t.net/addgene:186816 ; RRID:Addgene_186816)

  • For your References section:

    Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Oura S, Ninomiya A, Sugihara F, Matzuk MM, Ikawa M. Sci Rep. 2022 Dec 23;12(1):22198. doi: 10.1038/s41598-022-26501-7. 10.1038/s41598-022-26501-7 PubMed 36564444
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