pCAG-MCS-TurboID-3xFLAG
(Plasmid
#186814)
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Purpose(Empty Backbone) CAG promoter-driven expression of BioID2-fused protein (C terminus)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG1.1
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Backbone manufacturerMasahito Ikawa
- Backbone size (bp) 5182
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Vector typeMammalian Expression
- Promoter CAG promoter
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Tag
/ Fusion Protein
- TurboID-3xFLAG (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer 5'-gccttcttctttttcctacagc-3'
- 3′ sequencing primer 5'-ccagctgttcgccactatgg-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-MCS-TurboID-3xFLAG was a gift from Masahito Ikawa (Addgene plasmid # 186814 ; http://n2t.net/addgene:186814 ; RRID:Addgene_186814) -
For your References section:
Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Oura S, Ninomiya A, Sugihara F, Matzuk MM, Ikawa M. Sci Rep. 2022 Dec 23;12(1):22198. doi: 10.1038/s41598-022-26501-7. 10.1038/s41598-022-26501-7 PubMed 36564444
