pcDNA3.3_SARS2_BA.4/5
(Plasmid #186810)

• Purpose: pseudovirus plasmid expressing BA.4 and BA.5 spike protein
• Depositing Lab: David Nemazee
• Publication: Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773.

Backbone

• Vector backbone: pcDNA3.3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 9300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 43740568
• Species: SARS-CoV-2
• Insert Size (bp): 3800
• Mutation: HY69-70del, L452R, F486V, Q493R, on the basis of BA.2, c-terminal 18 aa del
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: TK_pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: fragment synthesized from Burton lab
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.04.11.487879v1.